Isolation, Cloning and Expression of Insecticidal-Protein-Encoding Gene tcdA from Photorhabdus luminescens in Escherichia coli
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منابع مشابه
Isolation, Cloning and Expression of Insecticidal-Protein-Encoding Gene tcdA from Photorhabdus luminescens in Escherichia coli
Entomopathogenic nematodes can provide effective biological control of several important soil insect pests that occur in cryptic habitats (Georgis and Manweiler, 1994; Koppenhofer, 2000). Favorable characteristics include formation of a durable infective stage that can be stored for long periods and applied by conventional methods, and persistence in the environment following application. In ad...
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Four toxin complexes, Tca, Tcb, Tcc and Tcd from the culture broth of Photorhabdus luminescens have been puri®ed and the four toxin complex encoding loci, tca, tcb, tcc and tcd, cloned. Genetic knockout of either tca or tcd reduced oral toxicity to Manduca sexta, and knockout of both loci eliminated activity. Puri®ed Tca speci®cally affected the insect midgut, despite its putative normal delive...
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ژورنال
عنوان ژورنال: International Journal of Current Microbiology and Applied Sciences
سال: 2017
ISSN: 2319-7692,2319-7706
DOI: 10.20546/ijcmas.2017.609.212